Difference between revisions of "Part:BBa K4361312"

 
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<ul>
 
<ul>
  
−
<li>.</li>
+
<li>C 106 > T, silent mutation</li>
 +
<li>G 571 > T, resulting in substitution G191C</li>
 +
<li>G 614 > T, resulting in substitution R205L</li>
 +
<li>C 687 > T, silent mutation</li>
 +
<li>T 825 > A, resulting in substitution D275E</li>
 
</ul>
 
</ul>
  

Revision as of 14:08, 22 September 2022


BlcR L66I

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R3 (Part:BBa_K4361218) and F3.3 L66I (Part:BBa_K4361221). For this mutant, the leucine in position 66 has been changed to isoleucine by mutating the CTC codon to ATT.

This mutant also contains the following nucleotide mutations outside of the targeted site:

  • C 106 > T, silent mutation
  • G 571 > T, resulting in substitution G191C
  • G 614 > T, resulting in substitution R205L
  • C 687 > T, silent mutation
  • T 825 > A, resulting in substitution D275E

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589