Difference between revisions of "Part:BBa K4361306"
Line 8: Line 8:
 
<ul>
 
<ul>
  
−
<li>.</li>
+
<li>A 800 deletion, resulting in a possible frameshift and extension of the protein</li>
 
</ul>
 
</ul>
  

Revision as of 13:36, 22 September 2022


BlcR A62K

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R7 (Part:BBa_K4361210) and F7.4 A62K (Part:BBa_K4361214). For this mutant, the alanine in position 62 has been changed to lysine by mutating the GCG codon to AAA.

This mutant also contains the following nucleotide mutations outside of the targeted site:

  • A 800 deletion, resulting in a possible frameshift and extension of the protein

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589