Difference between revisions of "Part:BBa K4223006"
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| − | The Maltose Binding Protein (MBP) tag Protein is 40kDa in size and encoded by the malE gene of e. coli K12. MBP can increase the solubility of fusion proteins overexpressed in bacteria, especially eukaryotic proteins. If the protein is expressed in bacteria, the MBP can fuse at the N or C terminus of the protein. The MBP fusion protein can be purified by cross-linked starch affinity chromatography in one step, and the bound fusion protein can be eluted with 10mM maltose in physiological buffer. If the MBP fusion fraction is to be removed, site-specific protease excision is available. Recombinant proteins labeled with the MBP tag can be detected by MBP antibody or expressed antibody specific to the target protein. The prokaryotic expression vector of MBP fusion protein has the advantages of high expression efficiency and easy purification. MBP is widely used as a fusion protein in modern molecular cloning. | + | The Maltose Binding Protein (MBP) tag Protein is 40kDa in size and encoded by the malE gene of e. coli K12. MBP can increase the solubility of fusion proteins overexpressed in bacteria, especially eukaryotic proteins. If the protein is expressed in bacteria, the MBP can fuse at the N or C terminus of the protein. The MBP fusion protein can be purified by cross-linked starch affinity chromatography in one step, and the bound fusion protein can be eluted with 10mM maltose in physiological buffer. If the MBP fusion fraction is to be removed, site-specific protease excision is available. Recombinant proteins labeled with the MBP tag can be detected by MBP antibody or expressed antibody specific to the target protein. The prokaryotic expression vector of MBP fusion protein has the advantages of high expression efficiency and easy purification. MBP is widely used as a fusion protein in modern molecular cloning.<br><br> |
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| + | <h3>operating principle</h3> | ||
| + | [[File:Operating Principle of Maltose Binding Protein (MBP).png|300px|]]<br> | ||
| + | '''Figure 1.Operating Principle of Maltose Binding Protein (MBP)''' | ||
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Latest revision as of 13:20, 22 September 2022
Maltose Binding Protein (MBP) Tag protein
The Maltose Binding Protein (MBP) tag Protein is 40kDa in size and encoded by the malE gene of e. coli K12. MBP can increase the solubility of fusion proteins overexpressed in bacteria, especially eukaryotic proteins. If the protein is expressed in bacteria, the MBP can fuse at the N or C terminus of the protein. The MBP fusion protein can be purified by cross-linked starch affinity chromatography in one step, and the bound fusion protein can be eluted with 10mM maltose in physiological buffer. If the MBP fusion fraction is to be removed, site-specific protease excision is available. Recombinant proteins labeled with the MBP tag can be detected by MBP antibody or expressed antibody specific to the target protein. The prokaryotic expression vector of MBP fusion protein has the advantages of high expression efficiency and easy purification. MBP is widely used as a fusion protein in modern molecular cloning.
operating principle
.png)
Figure 1.Operating Principle of Maltose Binding Protein (MBP)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 360
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]