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Revision as of 13:04, 22 September 2022


BlcR S61V

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R7 (Part:BBa_K4361210) and F7.1 S61V (Part:BBa_K4361211). For this mutant, the serine in position 61 has been changed to valine by mutating the AGT codon to GTG.

This mutant also contains the following nucleotide mutations outside of the targeted site:

  • C 705 > T, silent mutation

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589