Difference between revisions of "Part:BBa K4197001"

 
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Brick expressing DARPin at the surface of E. coli cell sortable by FACS
 
Brick expressing DARPin at the surface of E. coli cell sortable by FACS
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<html>
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<h2>Introduction</h2>
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<p>Xxxxx xxxxx xxxxx xxxxxx xxxx</p>
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<h2>Construction</h2>
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<p>Xxxxx xxxxx xxxxx xxxxxx xxxx</p>
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<div class="center">
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        <div class="thumb tnone">
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            <div class="thumbinner" style="width:50%;">
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                <a href="/File:T--Toulouse-INSA-UPS--Registry--Youn--CerberusCloning.png" class="image">
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                    <img alt="" src="/wiki/images/7/7e/T--Toulouse-INSA-UPS--Registry--Youn--CerberusCloning.png" width="100%" height=auto class="thumbimage" /></a>                  <div class="thumbcaption">
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                    <div class="magnify">
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                        <a href="/File:T--Toulouse-INSA-UPS--Registry--Youn--CerberusCloning.png" class="internal" title="Enlarge"></a>
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                    </div>
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                    <b>Figure 1: </b> <b>Xxxxxx</b> 
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                  Xxxxxxxxxxxxxxxxxxxxxxxxxxx.
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                </div>
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            </div>
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        </div>
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    </div>
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<h2>Xxxxxxxxx</h2>
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<p>Xxxxxxxxxxxxx</p>
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<div class="center">
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    <div class="thumb tnone">
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        <div class="thumbinner" style="width:80%;">
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            <a href="http://2018.igem.org/File:T--Toulouse-INSA-UPS--Team--Callum-Model-5step_dist.gif" class="image">
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                <img alt="" src="https://static.igem.org/mediawiki/2018/5/5b/T--Toulouse-INSA-UPS--Team--Callum-Model-5step_dist.gif" width="100%" height=auto class="thumbimage" /></a>                  <div class="thumbcaption">
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                <div class="magnify">
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                    <a href="http://2018.igem.org/File:T--Toulouse-INSA-UPS--Team--Callum-Model-5step_dist.gif" class="internal" title="Enlarge"></a>
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                </div>
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                <b>Figure 2: </b> <b>Xxxxxxxxxxxxx</b> 
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                Xxxxxxxxxxxxx.
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            </div>
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        </div>
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    </div>
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</div>
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<h2>titre 2</h2>
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<h3>Titre 3</h3>
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<p>Xxxxxxxxxx</p>
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<ul>
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    <li>Forward : TAAGAAGGAGATATACCATGGCGGAAGCGGGTATCACC</li>
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    <li>Reverse : CTCGAGTGCGGCCGCAAGCTTCGGATCGTCCTATGATGGAGG</li>
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</ul>
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<p>Xxxxxxxxxx</p>
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<ul>
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    <li>CForward : CGCGGCCGCTTCTAGAGCGGAAGCGGGTATCACC</li>
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    <li>Reverse : AGCGGCCGCTACTAGTCGGATCGTCCTATGATGGAGG</li>
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</ul>
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<h3>titre 3</h3>
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    <h4>Titre 4</h4>
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<p>Xxxxxx</p>
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<h4>Titre 4</h4>
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<p>xxxxxxx</p>
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<h2>Titre 2</h2>
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<p>Xxxxxx</p>
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<h2>References</h2>
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<ol>
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    <i>
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    <li>Morag E, Lapidot A, Govorko D, Lamed R, Wilchek M, Bayer EA, Shoham Y: Expression, purification, and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum. Applied and Environmental Microbiology 1995, 61:1980-1986.</li>
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    <li>Nogueira ES, Schleier T, Durrenberger M, Ballmer-Hofer K, Ward TR, Jaussi R: High-level secretion of recombinant full-length streptavidin in Pichia pastoris and its application to enantioselective catalysis. Protein Expr Purif 2014, 93:54-62. DOI: 10.1016/j.pep.2013.10.015.</li>
 +
    <li>Young TS, Schultz PG: Beyond the canonical 20 amino acids: expanding the genetic lexicon. J Biol Chem 2010, 285:11039-11044. DOI: 10.1074/jbc.R109.091306.</li>
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</i>
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</ol>
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</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:57, 22 September 2022


OmpA_DARPin fusion + mTagBFP

Brick expressing DARPin at the surface of E. coli cell sortable by FACS

Introduction

Xxxxx xxxxx xxxxx xxxxxx xxxx

Construction

Xxxxx xxxxx xxxxx xxxxxx xxxx

Figure 1: Xxxxxx Xxxxxxxxxxxxxxxxxxxxxxxxxxx.

Xxxxxxxxx

Xxxxxxxxxxxxx

Figure 2: Xxxxxxxxxxxxx Xxxxxxxxxxxxx.

titre 2

Titre 3

Xxxxxxxxxx

  • Forward : TAAGAAGGAGATATACCATGGCGGAAGCGGGTATCACC
  • Reverse : CTCGAGTGCGGCCGCAAGCTTCGGATCGTCCTATGATGGAGG

Xxxxxxxxxx

  • CForward : CGCGGCCGCTTCTAGAGCGGAAGCGGGTATCACC
  • Reverse : AGCGGCCGCTACTAGTCGGATCGTCCTATGATGGAGG

titre 3

Titre 4

Xxxxxx

Titre 4

xxxxxxx

Titre 2

Xxxxxx

References

  1. Morag E, Lapidot A, Govorko D, Lamed R, Wilchek M, Bayer EA, Shoham Y: Expression, purification, and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum. Applied and Environmental Microbiology 1995, 61:1980-1986.
  2. Nogueira ES, Schleier T, Durrenberger M, Ballmer-Hofer K, Ward TR, Jaussi R: High-level secretion of recombinant full-length streptavidin in Pichia pastoris and its application to enantioselective catalysis. Protein Expr Purif 2014, 93:54-62. DOI: 10.1016/j.pep.2013.10.015.
  3. Young TS, Schultz PG: Beyond the canonical 20 amino acids: expanding the genetic lexicon. J Biol Chem 2010, 285:11039-11044. DOI: 10.1074/jbc.R109.091306.
    4.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1526
    Illegal EcoRI site found at 1740
    Illegal XbaI site found at 1511
    Illegal XbaI site found at 1657
    Illegal PstI site found at 213
    Illegal PstI site found at 224
    Illegal PstI site found at 342
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1526
    Illegal EcoRI site found at 1740
    Illegal NheI site found at 1702
    Illegal PstI site found at 213
    Illegal PstI site found at 224
    Illegal PstI site found at 342
    Illegal NotI site found at 1518
    Illegal NotI site found at 2510
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1526
    Illegal EcoRI site found at 1740
    Illegal BglII site found at 1591
    Illegal BamHI site found at 1734
    Illegal XhoI site found at 2519
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1526
    Illegal EcoRI site found at 1740
    Illegal XbaI site found at 1511
    Illegal XbaI site found at 1657
    Illegal PstI site found at 213
    Illegal PstI site found at 224
    Illegal PstI site found at 342
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1526
    Illegal EcoRI site found at 1740
    Illegal XbaI site found at 1511
    Illegal XbaI site found at 1657
    Illegal PstI site found at 213
    Illegal PstI site found at 224
    Illegal PstI site found at 342
    Illegal AgeI site found at 329
    Illegal AgeI site found at 2307
  • 1000
    COMPATIBLE WITH RFC[1000]