Difference between revisions of "Part:BBa K4197011"

 
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IPTG unducible system to express DARPin at the surface of <i>E.coli</i>.  
 
IPTG unducible system to express DARPin at the surface of <i>E.coli</i>.  
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<html>
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<h2>Introduction</h2>
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<p>Xxxxx xxxxx xxxxx xxxxxx xxxx</p>
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<h2>Construction</h2>
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<p>Xxxxx xxxxx xxxxx xxxxxx xxxx</p>
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<div class="center">
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        <div class="thumb tnone">
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            <div class="thumbinner" style="width:50%;">
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                <a href="/File:T--Toulouse-INSA-UPS--Registry--Youn--CerberusCloning.png" class="image">
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                    <img alt="" src="/wiki/images/7/7e/T--Toulouse-INSA-UPS--Registry--Youn--CerberusCloning.png" width="100%" height=auto class="thumbimage" /></a>                  <div class="thumbcaption">
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                    <div class="magnify">
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                        <a href="/File:T--Toulouse-INSA-UPS--Registry--Youn--CerberusCloning.png" class="internal" title="Enlarge"></a>
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                    </div>
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                    <b>Figure 1: </b> <b>Xxxxxx</b> 
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                  Xxxxxxxxxxxxxxxxxxxxxxxxxxx.
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                </div>
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            </div>
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        </div>
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    </div>
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<h2>Xxxxxxxxx</h2>
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<p>Xxxxxxxxxxxxx</p>
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<div class="center">
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    <div class="thumb tnone">
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        <div class="thumbinner" style="width:80%;">
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            <a href="http://2018.igem.org/File:T--Toulouse-INSA-UPS--Team--Callum-Model-5step_dist.gif" class="image">
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                <img alt="" src="https://static.igem.org/mediawiki/2018/5/5b/T--Toulouse-INSA-UPS--Team--Callum-Model-5step_dist.gif" width="100%" height=auto class="thumbimage" /></a>                  <div class="thumbcaption">
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                <div class="magnify">
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                    <a href="http://2018.igem.org/File:T--Toulouse-INSA-UPS--Team--Callum-Model-5step_dist.gif" class="internal" title="Enlarge"></a>
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                </div>
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                <b>Figure 2: </b> <b>Xxxxxxxxxxxxx</b> 
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                Xxxxxxxxxxxxx.
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            </div>
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        </div>
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    </div>
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</div>
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<h2>titre 2</h2>
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<h3>Titre 3</h3>
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<p>Xxxxxxxxxx</p>
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<ul>
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    <li>Forward : TAAGAAGGAGATATACCATGGCGGAAGCGGGTATCACC</li>
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    <li>Reverse : CTCGAGTGCGGCCGCAAGCTTCGGATCGTCCTATGATGGAGG</li>
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</ul>
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<p>Xxxxxxxxxx</p>
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<ul>
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    <li>CForward : CGCGGCCGCTTCTAGAGCGGAAGCGGGTATCACC</li>
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    <li>Reverse : AGCGGCCGCTACTAGTCGGATCGTCCTATGATGGAGG</li>
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</ul>
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<h3>titre 3</h3>
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    <h4>Titre 4</h4>
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<p>Xxxxxx</p>
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<h4>Titre 4</h4>
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<p>xxxxxxx</p>
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<h2>Titre 2</h2>
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<p>Xxxxxx</p>
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<h2>References</h2>
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<ol>
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    <i>
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    <li>Morag E, Lapidot A, Govorko D, Lamed R, Wilchek M, Bayer EA, Shoham Y: Expression, purification, and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum. Applied and Environmental Microbiology 1995, 61:1980-1986.</li>
 +
    <li>Nogueira ES, Schleier T, Durrenberger M, Ballmer-Hofer K, Ward TR, Jaussi R: High-level secretion of recombinant full-length streptavidin in Pichia pastoris and its application to enantioselective catalysis. Protein Expr Purif 2014, 93:54-62. DOI: 10.1016/j.pep.2013.10.015.</li>
 +
    <li>Young TS, Schultz PG: Beyond the canonical 20 amino acids: expanding the genetic lexicon. J Biol Chem 2010, 285:11039-11044. DOI: 10.1074/jbc.R109.091306.</li>
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</i>
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</ol>
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</html>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:48, 21 September 2022


OmpA_DARPin

IPTG unducible system to express DARPin at the surface of E.coli.

Introduction

Xxxxx xxxxx xxxxx xxxxxx xxxx

Construction

Xxxxx xxxxx xxxxx xxxxxx xxxx

Figure 1: Xxxxxx Xxxxxxxxxxxxxxxxxxxxxxxxxxx.

Xxxxxxxxx

Xxxxxxxxxxxxx

Figure 2: Xxxxxxxxxxxxx Xxxxxxxxxxxxx.

titre 2

Titre 3

Xxxxxxxxxx

  • Forward : TAAGAAGGAGATATACCATGGCGGAAGCGGGTATCACC
  • Reverse : CTCGAGTGCGGCCGCAAGCTTCGGATCGTCCTATGATGGAGG

Xxxxxxxxxx

  • CForward : CGCGGCCGCTTCTAGAGCGGAAGCGGGTATCACC
  • Reverse : AGCGGCCGCTACTAGTCGGATCGTCCTATGATGGAGG

titre 3

Titre 4

Xxxxxx

Titre 4

xxxxxxx

Titre 2

Xxxxxx

References

  1. Morag E, Lapidot A, Govorko D, Lamed R, Wilchek M, Bayer EA, Shoham Y: Expression, purification, and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum. Applied and Environmental Microbiology 1995, 61:1980-1986.
  2. Nogueira ES, Schleier T, Durrenberger M, Ballmer-Hofer K, Ward TR, Jaussi R: High-level secretion of recombinant full-length streptavidin in Pichia pastoris and its application to enantioselective catalysis. Protein Expr Purif 2014, 93:54-62. DOI: 10.1016/j.pep.2013.10.015.
  3. Young TS, Schultz PG: Beyond the canonical 20 amino acids: expanding the genetic lexicon. J Biol Chem 2010, 285:11039-11044. DOI: 10.1074/jbc.R109.091306.
    4.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 130
    Illegal XbaI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 130
    Illegal NheI site found at 92
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 130
    Illegal BamHI site found at 124
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 130
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 130
    Illegal XbaI site found at 47
    Illegal AgeI site found at 832
  • 1000
    COMPATIBLE WITH RFC[1000]