Difference between revisions of "Part:BBa K4115002"

 
Line 7: Line 7:
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
−
 
+
<br/>
 +
The nifA protein encoded by the nifA sequence is a transcriptional activator that can activate the transcription of the downstream nifH promoter under the influence of cytosolic nitrogen sources or oxygen, and is a key activator of nitrogen-fixing gene clusters. The nitrogen fixation gene can be regulated by knocking out the native nifA sequence and replacing it with the nifA sequence added with regulatory elements.
 +
<br/>
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 14:07, 21 September 2022


NifA(nif-specific transcriptional activator)

The coding sequence of the nifA protein in the nitrogen-fixing gene cluster.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 136
    Illegal PstI site found at 1000
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 136
    Illegal PstI site found at 1000
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 637
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 136
    Illegal PstI site found at 1000
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 136
    Illegal PstI site found at 1000
    Illegal NgoMIV site found at 1257
    Illegal NgoMIV site found at 1452
    Illegal NgoMIV site found at 1527
    Illegal AgeI site found at 886
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1095
    Illegal SapI site found at 318
    Illegal SapI site found at 939