Difference between revisions of "Part:BBa K4204000"

 
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LysPBC5 is an endolysin that specifically lyse Bacillus Cereus but also has some activity against other strains in B.cereus group. It is composed of two domains: a CBD (cell-wall binding domain) that recognizes and binds to specific cell wall peptidoglycan (PG)  structures, and the EAD (enzymatically active domain) that later cleaves the glycosidic bonds in PG layers to break the cell wall [1].
 
LysPBC5 is an endolysin that specifically lyse Bacillus Cereus but also has some activity against other strains in B.cereus group. It is composed of two domains: a CBD (cell-wall binding domain) that recognizes and binds to specific cell wall peptidoglycan (PG)  structures, and the EAD (enzymatically active domain) that later cleaves the glycosidic bonds in PG layers to break the cell wall [1].
  
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[1] Lee, K. O., Kong, M., Kim, I., Bai, J., Cha, S., Kim, B., Ryu, K.-S., Ryu, S., & Suh, J.-Y. (2019). Structural basis for cell-wall recognition by bacteriophage PBC5 endolysin. Structure, 27(9). https://doi.org/10.1016/j.str.2019.07.001
 
  
  

Revision as of 11:10, 20 September 2022


Coding sequence of endolysin LysPBC5

This part only contains the ORF of LysPBC5.

LysPBC5 is an endolysin that specifically lyse Bacillus Cereus but also has some activity against other strains in B.cereus group. It is composed of two domains: a CBD (cell-wall binding domain) that recognizes and binds to specific cell wall peptidoglycan (PG) structures, and the EAD (enzymatically active domain) that later cleaves the glycosidic bonds in PG layers to break the cell wall [1].



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 690
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 742
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 988