Difference between revisions of "Part:BBa K4361303"

 
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<partinfo>BBa_K4361303 short</partinfo>
 
<partinfo>BBa_K4361303 short</partinfo>
  
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A mutant of the BlcR protein, created through site-directed mutagenesis with primers R4 ([[Part:BBa_K4361204]]) and F4.2 G41A (<b>TBD</b>). For this mutant, the glycine in position 41 has been changed to alanine by mutating the GGC codon to GCG.
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A mutant of the BlcR protein, created through site-directed mutagenesis with primers R7 ([[Part:BBa_K4361210]]) and F7.1 S61V ([[Part:BBa_K4361211]]). For this mutant, the serine in position 61 has been changed to valine by mutating the AGT codon to GTG.
  
 
This mutant also contains the following nucleotide mutations outside of the targeted site:
 
This mutant also contains the following nucleotide mutations outside of the targeted site:

Revision as of 12:27, 19 September 2022


BlcR S61V

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R7 (Part:BBa_K4361210) and F7.1 S61V (Part:BBa_K4361211). For this mutant, the serine in position 61 has been changed to valine by mutating the AGT codon to GTG.

This mutant also contains the following nucleotide mutations outside of the targeted site:

  • .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589