Difference between revisions of "Part:BBa K4158006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | If you want to get same plasmid that we experimented with, construct the plasmid along this way below. | |
| + | 1. Prepare pJL1 cloning vector (addgene Plasmid #69496)and this part as BioBrick. | ||
| + | 2. Restriction and insertion cloning with pJL1 and this BioBrick productions and restriction enzymes XbaI and salI. | ||
===Source=== | ===Source=== | ||
Revision as of 06:47, 18 September 2022
RBS-AtzB
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1087
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1087
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 338
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1087
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1087
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
If you want to get same plasmid that we experimented with, construct the plasmid along this way below.
1. Prepare pJL1 cloning vector (addgene Plasmid #69496)and this part as BioBrick.
2. Restriction and insertion cloning with pJL1 and this BioBrick productions and restriction enzymes XbaI and salI.
Source
test