Difference between revisions of "Part:BBa K4308000"

Revision as of 14:29, 14 September 2022

Forward Primer for RPA of LINC00857

Long intergenic non-protein coding RNA 857 (LINC00857) is a genetic molecule involved in cancer biology, which is identified as one of the most upregulated LINCRNAs in lung cancer. It could lead to resistance to platinum‐induced apoptosis by affecting mRNA stability or promoting its translation. Thus, we chose LINC00857 as a novel prognostic and cancer predictive biomarker. We use this part as the forward primer to conduct recombinase-aid amplification (RAA) of LINC00857, along with its reverse primer (BBa_K4308001).

Usage and Biology

In our project, this primer (BBa_K4308000) was used along with its reverse primer (BBa_K4308001) for the amplification reactions of LINC00857, in order to perform isothermal amplification reactions with recombinase-aid amplification (RAA). Using this method, we were able to detect quantities as low as 10^-9 nanograms during 30 minutes. The expected amplified product length is about 150 bp.

RAA reaction after clean up of the amplified product:

Sequence and Features