Difference between revisions of "Part:BBa K4361201"
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For the site-directed mutagenesis (SDM) of our protein of interest, BlcR, multiple sets of back-to-back primers were designed. The 5' ends of these primers anneal to adjacent nucleotides such that the full plasmid containing the protein ([http://www.parts.igem.org/Part:BBa_K4361106/ BBa_K4361106]) can be replicated. Each of these sets of primers contains a single reverse primer, denoted as "primer R<i>n</i>" with <i>n</i> being the number of the set, in this case primer R1 ([https://parts.igem.org/Part:BBa_K4361200 BBa_K4361200]), and a number of forward primers including this BioBrick. This set covers amino acids 36 to 39, although 36 is not represented in our BioBricks due to that SDM experiment being unsuccessful.<br> | For the site-directed mutagenesis (SDM) of our protein of interest, BlcR, multiple sets of back-to-back primers were designed. The 5' ends of these primers anneal to adjacent nucleotides such that the full plasmid containing the protein ([http://www.parts.igem.org/Part:BBa_K4361106/ BBa_K4361106]) can be replicated. Each of these sets of primers contains a single reverse primer, denoted as "primer R<i>n</i>" with <i>n</i> being the number of the set, in this case primer R1 ([https://parts.igem.org/Part:BBa_K4361200 BBa_K4361200]), and a number of forward primers including this BioBrick. This set covers amino acids 36 to 39, although 36 is not represented in our BioBricks due to that SDM experiment being unsuccessful.<br> | ||
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| − | This primer contains a mutation that changes the codon for the aspartic acid (D, GAC) in position 37 to that of arginine (R, CGC). | + | This primer contains a mutation that changes the codon for the aspartic acid (D, GAC) in position 37 to that of arginine (R, CGC). <br> |
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Revision as of 08:38, 12 September 2022
BlcR BTB primer F1.1 D37R
For the site-directed mutagenesis (SDM) of our protein of interest, BlcR, multiple sets of back-to-back primers were designed. The 5' ends of these primers anneal to adjacent nucleotides such that the full plasmid containing the protein ([http://www.parts.igem.org/Part:BBa_K4361106/ BBa_K4361106]) can be replicated. Each of these sets of primers contains a single reverse primer, denoted as "primer Rn" with n being the number of the set, in this case primer R1 (BBa_K4361200), and a number of forward primers including this BioBrick. This set covers amino acids 36 to 39, although 36 is not represented in our BioBricks due to that SDM experiment being unsuccessful.
This primer contains a mutation that changes the codon for the aspartic acid (D, GAC) in position 37 to that of arginine (R, CGC).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]