Difference between revisions of "Part:BBa K4235000:Design"
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| − | ===Design=== | + | ===Design and cloning information=== |
PROS1, the homo sapiens protein S gene, (NCBI:5627) is found on chromosome 3 and is known to exist in two major variants: the transcript variant 1 (NM_001314077.2) has an aligned length of 3,468 bp, the CDS length is 2,127 bp resulting in a 708 aa protein. The transcript variant 2 (NM_000313.4) has an aligned length of 3,372 bp, the CDS length is 2,031 bp resulting in a 676 aa protein. This part is designed from the 2,031 bp coding DNA sequence of the homo sapiens PROS1 gene, purposefully leaving out the 14 introns to streamline transcription and avoid undesired alternative splicing transcripts. The vertebrate Kozak sequence was also added to the 5’ end of the gene before synthesis. [also add primer info] | PROS1, the homo sapiens protein S gene, (NCBI:5627) is found on chromosome 3 and is known to exist in two major variants: the transcript variant 1 (NM_001314077.2) has an aligned length of 3,468 bp, the CDS length is 2,127 bp resulting in a 708 aa protein. The transcript variant 2 (NM_000313.4) has an aligned length of 3,372 bp, the CDS length is 2,031 bp resulting in a 676 aa protein. This part is designed from the 2,031 bp coding DNA sequence of the homo sapiens PROS1 gene, purposefully leaving out the 14 introns to streamline transcription and avoid undesired alternative splicing transcripts. The vertebrate Kozak sequence was also added to the 5’ end of the gene before synthesis. [also add primer info] | ||
[talk about LIC cloning] | [talk about LIC cloning] | ||
Revision as of 21:24, 10 September 2022
Design and cloning information
PROS1, the homo sapiens protein S gene, (NCBI:5627) is found on chromosome 3 and is known to exist in two major variants: the transcript variant 1 (NM_001314077.2) has an aligned length of 3,468 bp, the CDS length is 2,127 bp resulting in a 708 aa protein. The transcript variant 2 (NM_000313.4) has an aligned length of 3,372 bp, the CDS length is 2,031 bp resulting in a 676 aa protein. This part is designed from the 2,031 bp coding DNA sequence of the homo sapiens PROS1 gene, purposefully leaving out the 14 introns to streamline transcription and avoid undesired alternative splicing transcripts. The vertebrate Kozak sequence was also added to the 5’ end of the gene before synthesis. [also add primer info] [talk about LIC cloning]