Difference between revisions of "Part:BBa K143059"

 
m
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K143059 short</partinfo>
 
<partinfo>BBa_K143059 short</partinfo>
 +
 +
  
 
Sigma B dependant promoter ctc(<bbpart>BBa_K143010</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from ''B. subtilis''.
 
Sigma B dependant promoter ctc(<bbpart>BBa_K143010</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from ''B. subtilis''.
 +
  
 
In ''B. subtilis'' endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol<cite>1</cite>. The promoter ctc has been used previously as a read out for the activation of sigma factor B <cite>2</cite>.
 
In ''B. subtilis'' endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol<cite>1</cite>. The promoter ctc has been used previously as a read out for the activation of sigma factor B <cite>2</cite>.
  
−
Pctc has been used to take an input of blue light and give a '''Ribosomes per second'''(RiPS) output. To work as a blue light receiver correctly, over-expression of the blue light receptor '''YtvA''' (<bbpart>BBa_K143037</bbpart>) is required.
 
  
−
'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix<cite>1</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''  
+
Pctc may potentially be used to take an input of blue light and give a '''Ribosomes per second'''(RiPS) output. To work as a blue light receiver correctly, '''significant''' over-expression of the blue light receptor '''YtvA''' (<bbpart>BBa_K143037</bbpart>) is required.
 +
 
 +
 
 +
'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix<cite>3</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''  
  
  
Line 16: Line 20:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'><big>'''Sequence and Features'''</big></span>
 +
 
 +
 
 
<partinfo>BBa_K143059 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143059 SequenceAndFeatures</partinfo>
  
 +
===References===
 +
<biblio>
 +
#1 pmid=3100810
 +
#2 pmid=17575448
 +
#3 https://parts.igem.org/Assembly:RBS-CDS_issues
 +
<biblio>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 11:08, 30 July 2009

Promoter ctc and RBS spoVG for B. subtilis


Sigma B dependant promoter ctc(BBa_K143010) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis.


In B. subtilis endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol1. The promoter ctc has been used previously as a read out for the activation of sigma factor B 2.


Pctc may potentially be used to take an input of blue light and give a Ribosomes per second(RiPS) output. To work as a blue light receiver correctly, significant over-expression of the blue light receptor YtvA (BBa_K143037) is required.


To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix3. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

<biblio>

  1. 1 pmid=3100810
  2. 2 pmid=17575448
  3. 3 https://parts.igem.org/Assembly:RBS-CDS_issues

<biblio>