Difference between revisions of "Part:BBa K176064:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K176064 short</partinfo>
 
<partinfo>BBa_K176064 short</partinfo>
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Design:
 
Design:
  
−
pCon 0.70(J23101) generates TetR-LVA
+
pCon 0.70(J23101) generates TetR(no LVA)
  
 
pCon 0.70(J23101) generates LuxR
 
pCon 0.70(J23101) generates LuxR
  
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pLux/Tet(K176000) generates PoPS, activated by LuxR and Input2 AHL, repressed by TetR-LVA and no Input1 aTc
+
pLux/Tet(K176000) generates PoPS, activated by LuxR and Input2 AHL, repressed by TetR and no Input1 aTc
  
  

Latest revision as of 07:05, 30 July 2009

aTc&AHL->PoPS: pCon 0.70->tetR+pCon 0.70->luxR+pLux/Tet->PoPS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 840
    Illegal NheI site found at 863
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1818


Design Notes

Input1: aTc

Input2: AHL

Output: PoPS

Design:

pCon 0.70(J23101) generates TetR(no LVA)

pCon 0.70(J23101) generates LuxR

pLux/Tet(K176000) generates PoPS, activated by LuxR and Input2 AHL, repressed by TetR and no Input1 aTc


Source

J23101 K176031

References