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| Line 39: |
Line 39: |
| | | Hairpin loop || 3.00 || Closing pair is t<sub>12</sub>-a<sub>17</sub> | | | Hairpin loop || 3.00 || Closing pair is t<sub>12</sub>-a<sub>17</sub> |
| | |} | | |} |
| − | |}
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| − |
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| − |
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| − |
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| − | {| class="wikitable" style="margin:auto"
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| − | |+ Loop Free-Energy Decomposition
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| − | |-
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| − | ! Structural element !! δG !! Information
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| − | |-
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| − | | External loop || -0.86 || 15 ss bases & 1 closing helices
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| − | |-
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| − | | Stack || -1.00 || External closing pair is a<sub>4</sub>-t<sub>25</sub>
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| − | |-
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| − | | Stack || -1.44 || External closing pair is a<sub>5</sub>-t<sub>24</sub>
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| − | |-
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| − | | Stack || -2.17 || External closing pair is c<sub>6</sub>-g<sub>23</sub>
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| − | |-
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| − | | Stack || -1.84 || External closing pair is g<sub>7</sub>-c<sub>22</sub>
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| − | |-
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| − | | Stack || -2.24 || External closing pair is g<sub>8</sub>-c<sub>21</sub>
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| − | |-
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| − | | Stack || -1.84 || External closing pair is c<sub>9</sub>-g<sub>20</sub>
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| − | |-
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| − | | Stack || -1.84 || External closing pair is c<sub>10</sub>-g<sub>19</sub>
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| − | |-
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| − | | Stack || -1.28 || External closing pair is c<sub>11</sub>-g<sub>18</sub>
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| − | |-
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| − | ! Helix !! -13.65 !! 9 base pairs
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| − | |-
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| − | | Hairpin loop || 3.00 || Closing pair is t<sub>12</sub>-a<sub>17</sub>
| |
| | |} | | |} |
| | | | |
Revision as of 14:41, 5 September 2022
L2U2H09 Terminator
L2U2H09 is a synthetic terminator. The origin is https://www.addgene.org/127042/
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L2U2H09 structural information
Loop Free-Energy Decomposition
| Structural element |
δG |
Information
|
| External loop |
-0.86 |
15 ss bases & 1 closing helices
|
| Stack |
-1.00 |
External closing pair is a4-t25
|
| Stack |
-1.44 |
External closing pair is a5-t24
|
| Stack |
-2.17 |
External closing pair is c6-g23
|
| Stack |
-1.84 |
External closing pair is g7-c22
|
| Stack |
-2.24 |
External closing pair is g8-c21
|
| Stack |
-1.84 |
External closing pair is c9-g20
|
| Stack |
-1.84 |
External closing pair is c10-g19
|
| Stack |
-1.28 |
External closing pair is c11-g18
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| Helix |
-13.65 |
9 base pairs
|
| Hairpin loop |
3.00 |
Closing pair is t12-a17
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Sequence and Features
Assembly Compatibility:
- 10
- 12
- 21
- 23
- 25
- 1000
COMPATIBLE WITH RFC[1000]
