Difference between revisions of "Part:BBa K4390027:Design"

 
(→‎Design Notes)
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This compound (level 1) part was assembled using JUMP, a type IIS assembly method, so all basic (level 0) parts had to have the correct JUMP fusion sites flanking, as well as BsaI sites for level 1 assembly and BsmBI sites for level 0 assembly.
 
This compound (level 1) part was assembled using JUMP, a type IIS assembly method, so all basic (level 0) parts had to have the correct JUMP fusion sites flanking, as well as BsaI sites for level 1 assembly and BsmBI sites for level 0 assembly.
  
−
 
+
{| class="wikitable" style="margin:auto"
−
 
+
|+ JUMP fusion sites
−
 
+
|-
 +
! Part type !! 5’ Fusion site !! 3’ Fusion site
 +
|-
 +
| P (Promoter) || GGAG || TACT
 +
|-
 +
| R (Ribosome Binding Site) || TACT || AATG
 +
|-
 +
| N (N-terminus) || AATG || AGCC
 +
|-
 +
| O (Open Reading Frame)|| AGCC || TTCG
 +
|-
 +
| C (C-terminus)|| TTCG || GCTT
 +
|-
 +
| T (Terminator)|| GGCT || CGCT
 +
|}
  
 
===Source===
 
===Source===

Revision as of 17:46, 4 September 2022


SB7-tagged sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
    Illegal NotI site found at 73
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 111


Design Notes

This compound (level 1) part was assembled using JUMP, a type IIS assembly method, so all basic (level 0) parts had to have the correct JUMP fusion sites flanking, as well as BsaI sites for level 1 assembly and BsmBI sites for level 0 assembly.

JUMP fusion sites
Part type 5’ Fusion site 3’ Fusion site
P (Promoter) GGAG TACT
R (Ribosome Binding Site) TACT AATG
N (N-terminus) AATG AGCC
O (Open Reading Frame) AGCC TTCG
C (C-terminus) TTCG GCTT
T (Terminator) GGCT CGCT

Source

-

References