Difference between revisions of "Part:BBa K4348000:Design"

 
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===Design Notes===
 
===Design Notes===
−
An extra guanidine residue was added to the his tag to include an NcoI site.
 
 
Amino acid sequence was codon optimized for expression in E. coli
 
Amino acid sequence was codon optimized for expression in E. coli
−
 
+
One of the codons needed to be changed to remove an EcoRI site
−
 
+
  
 
===Source===
 
===Source===
  
−
Genomic sequence from Eubacterium coprostanoligenes
+
Genomic sequence from human
  
 
===References===
 
===References===

Latest revision as of 17:53, 22 August 2022


AKR1D1_his


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amino acid sequence was codon optimized for expression in E. coli One of the codons needed to be changed to remove an EcoRI site

Source

Genomic sequence from human

References