Difference between revisions of "Part:BBa K4370005:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The sequence of the native gene STRBO_RS0123570 of <i> Streptomyces bottropensis </i> ATCC 25435 has been codon optimized for an expression in <i> E. coli </i>. Indeed, the <i> Streptomyces</i> genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in <i> E. coli </i> | + | The sequence of the native gene STRBO_RS0123570 of <i> Streptomyces bottropensis </i> ATCC 25435 has been codon optimized for an expression in <i> E. coli </i>. Indeed, the <i> Streptomyces</i> genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in <i> E. coli </i>. |
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
+ | Reference of <i> Streptomyces bottropensis </i> ATCC 25435 genome: GCF_000383595.1_ASM38359v1 |
Latest revision as of 07:21, 22 August 2022
RBS_PRK_STRBO
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence of the native gene STRBO_RS0123570 of Streptomyces bottropensis ATCC 25435 has been codon optimized for an expression in E. coli . Indeed, the Streptomyces genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in E. coli .
Source
This sequence has been synthetized.
References
Reference of Streptomyces bottropensis ATCC 25435 genome: GCF_000383595.1_ASM38359v1