Difference between revisions of "Part:BBa K4370000:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The sequence of the native gene STRBO_RS0123595 of Streptomyces bottropensis ATCC 25435 has been codon optimized for an expression in E. coli. Indeed, the Streptomyces genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in E. coli. | + | The sequence of the native gene STRBO_RS0123595 of <i> Streptomyces bottropensis </i> ATCC 25435 has been codon optimized for an expression in <i> E. coli </i>. Indeed, the <i> Streptomyces genomes </i> are GC rich (circa 72%), which requires extensive sequence optimization for expression in <i> E. coli </i>. |
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===Source=== | ===Source=== | ||
− | This sequence has | + | This sequence has been synthetized. |
===References=== | ===References=== | ||
+ | Reference of <i> Streptomyces bottropensis </i> ATCC 25435 genome: GCF_000383595.1_ASM38359v1 |
Latest revision as of 07:14, 22 August 2022
cbbL_STRBO
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 373
Design Notes
The sequence of the native gene STRBO_RS0123595 of Streptomyces bottropensis ATCC 25435 has been codon optimized for an expression in E. coli . Indeed, the Streptomyces genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in E. coli .
Source
This sequence has been synthetized.
References
Reference of Streptomyces bottropensis ATCC 25435 genome: GCF_000383595.1_ASM38359v1