Difference between revisions of "Part:BBa K4370010:Design"
Line 16: | Line 16: | ||
===References=== | ===References=== | ||
− | Bai | + | Chaoxian Bai , Yang Zhang , Xuejin Zhao, Yiling Hu, Sihai Xiang, Jin Miao, Chunbo Lou, Lixin Zhang “Exploiting a precise design of universal synthetic modular regulatory elements to unlock the microbial natural products in <i>Streptomyces</i>”, Proc Natl Acad Sci U S A , 2015 Sep 29;112(39):12181-6. https://www.pnas.org/doi/10.1073/pnas.1511027112 |
− | Wang | + | Weishan Wang, Xiao Li, Juan Wang, Sihai Xiang, Xiaozhou Feng, Keqian Yang, “An engineered strong promoter for <i>Streptomycetes</i>”, Appl Environ Microbiol., 2013 Jul;79(14):4484-92. |
+ | https://doi.org/10.1128/AEM.00985-13 | ||
− | Aubry | + | Céline Aubry, Jean-Luc Pernodet , Sylvie Lautru, « Modular and Integrative Vectors for Synthetic Biology Applications in <i>Streptomyces</i> spp”, Appl Environ Microbiol, 2019 Aug 1;85(16):e00485-19 |
+ | https://doi.org/10.1128/AEM.00485-19 |
Revision as of 17:03, 21 August 2022
STREPTO_E.coli_CLONING_MODULE
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 792
Illegal AgeI site found at 904 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The NdeI site (catATG) allows the easy cloning of genes between NdeI (ATG/start codon) and SpeI sites (this latter site been in the suffixe). This module is also designed to be easily cloned between NotI sites in the collection of Streptomyces integrative vectors published by Aubry and collaborators (Appl Environ Microbiol, 2019).
Source
The sequence has been synthetized.
References
Chaoxian Bai , Yang Zhang , Xuejin Zhao, Yiling Hu, Sihai Xiang, Jin Miao, Chunbo Lou, Lixin Zhang “Exploiting a precise design of universal synthetic modular regulatory elements to unlock the microbial natural products in Streptomyces”, Proc Natl Acad Sci U S A , 2015 Sep 29;112(39):12181-6. https://www.pnas.org/doi/10.1073/pnas.1511027112
Weishan Wang, Xiao Li, Juan Wang, Sihai Xiang, Xiaozhou Feng, Keqian Yang, “An engineered strong promoter for Streptomycetes”, Appl Environ Microbiol., 2013 Jul;79(14):4484-92. https://doi.org/10.1128/AEM.00985-13
Céline Aubry, Jean-Luc Pernodet , Sylvie Lautru, « Modular and Integrative Vectors for Synthetic Biology Applications in Streptomyces spp”, Appl Environ Microbiol, 2019 Aug 1;85(16):e00485-19 https://doi.org/10.1128/AEM.00485-19