Difference between revisions of "Part:BBa K4245000"

 
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<partinfo>BBa_K4245000 short</partinfo>
 
<partinfo>BBa_K4245000 short</partinfo>
  
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BBa_K4245000 is a composite part producing a fluorescent RNA aptamer called Spinach. As shown in Figure 3, BBa_K4245000 consists of an inducible promoter (BBa_R0010) that is repressed by the LacI gene, a Spinach Aptamer (BBa_K734002) with stabilizing tRNA scaffolds designed by Austin Texas iGEM 2012, and a T7 terminator (BBa_K731721).
 
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The LacI protein represses the inducible promoter (BBa_R0010), thus stopping downstream transcription of the Spinach aptamer (BBa_K734002). When IPTG is present, it inhibits LacI from repressing the promoter, allowing transcription of the aptamer. Once DFHBI (3,5-difluoro-4-hydroxybenzylidene imidazolinone) binds to the aptamer, quantifiable green fluorescence is produced.
 
  
 
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Revision as of 00:14, 18 August 2022


iSpinach-D5-G30-A32 Aptamer


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]