Difference between revisions of "Part:BBa K4370005:Design"

 
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===Design Notes===
 
===Design Notes===
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The sequence of the native gene STRBO_RS0123570 of <i> Streptomyces bottropensis </i> ATCC 25435 has been codon optimized for an expression in <i> E. coli </i>. Indeed, the <i> Streptomyces</i> genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in <i> E. coli </i>.
+
The sequence of the native gene STRBO_RS0123570 of <i> Streptomyces bottropensis </i> ATCC 25435 has been codon optimized for an expression in <i> E. coli </i>. Indeed, the <i> Streptomyces</i> genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in <i> E. coli </i>. For our project, the sequence has being cloned in pBAD33 by Golden Gate Assembly.
  
 
Source
 
Source

Revision as of 15:37, 17 August 2022


RBS_PRK_STRBO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of the native gene STRBO_RS0123570 of Streptomyces bottropensis ATCC 25435 has been codon optimized for an expression in E. coli . Indeed, the Streptomyces genomes are GC rich (circa 72%), which requires extensive sequence optimization for expression in E. coli . For our project, the sequence has being cloned in pBAD33 by Golden Gate Assembly.

Source


Source

This sequence has been synthetized.

References