Difference between revisions of "Part:BBa K4150000"

Revision as of 08:38, 11 August 2022

g10.RBS

ThisRBS contains a g10 leader sequence from T7 bacteriophage gene 10, which encodes phage coat protein at very high expression level^[1].

↑ Olins PO, Devine CS, Rangwala SH, Kavka KS. The T7 phage gene 10 leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in Escherichia coli. Gene. 1988 Dec 15;73(1):227-35. doi: 10.1016/0378-1119(88)90329-0. PMID: 3072257.

Assembly Compatibility:

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 <partinfo>BBa_K4150000 short</partinfo>
-<span style="color:#00000000">This</span>RBS contains a g10 leader sequence from T7 bacteriophage gene 10.
+<span style="color:#00000000">This</span>RBS contains a g10 leader sequence from T7 bacteriophage gene 10, which encodes phage coat protein at very high expression level<ref>Olins PO, Devine CS, Rangwala SH, Kavka KS. The T7 phage gene 10 leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in Escherichia coli. Gene. 1988 Dec 15;73(1):227-35. doi: 10.1016/0378-1119(88)90329-0. PMID: 3072257.</ref>.
-<ref>Kim S, Kim M, Ryu S. Development of an engineered bioluminescent reporter phage for the sensitive detection of viable Salmonella typhimurium. Anal Chem. 2014 Jun 17;86(12):5858-64. doi: 10.1021/ac500645c.</ref>