Difference between revisions of "Part:BBa K4150004"

 
Line 3: Line 3:
 
<partinfo>BBa_K4150004 short</partinfo>
 
<partinfo>BBa_K4150004 short</partinfo>
  
−
The chicken ovalbumin sequence obtained from KEGG database ([https://www.genome.jp/entry/gga:396058 Gallus gallus]) was synthesized by Integrated DNA Technologies, Inc. and connected with a His tag and a GS linker at N-terminus. The ovalbumin (OVA) is a glycoprotein, which is a major component of chicken egg whites, and harbors immunogenic properties in vaccination experiments. The recombinant OVA protein is readily purified in E. coli BL21 system driven by T7 promoter and triggered by IPTG induction.     
+
The chicken ovalbumin sequence obtained from KEGG database ([https://www.genome.jp/entry/gga:396058 Gallus gallus]) was codon optimzed for expression in E. coli and synthesized by Integrated DNA Technologies, Inc. with a connection of a His tag and a GS linker at N-terminus. The ovalbumin (OVA) is a glycoprotein, which is a major component of chicken egg whites, and harbors immunogenic properties in vaccination experiments. The recombinant OVA protein is readily purified in E. coli BL21 system driven by T7 promoter and triggered by IPTG induction.     
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 9: Line 9:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
===<span class='h3bb'>Sequence and Features</span>===
 
<partinfo>BBa_K4150004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4150004 SequenceAndFeatures</partinfo>
  

Revision as of 08:17, 11 August 2022


His-OVA

The chicken ovalbumin sequence obtained from KEGG database (Gallus gallus) was codon optimzed for expression in E. coli and synthesized by Integrated DNA Technologies, Inc. with a connection of a His tag and a GS linker at N-terminus. The ovalbumin (OVA) is a glycoprotein, which is a major component of chicken egg whites, and harbors immunogenic properties in vaccination experiments. The recombinant OVA protein is readily purified in E. coli BL21 system driven by T7 promoter and triggered by IPTG induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 373