Difference between revisions of "Part:BBa K4390023:Design"

 
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===Design Notes===
 
===Design Notes===
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This part was designed to be used in JUMP assembly as an O part, so the last four nucleotides in the glycine-serine linker have been removed, as they will be regenerated by the O part 3' fusion site (TTCG), creating a scarless final assembly. To use this sequence in non-JUMP assembly, add TTCG to the end of the sequence.
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Revision as of 17:52, 7 August 2022


Cellulose Binding Domain :: Gly-Ser Linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to be used in JUMP assembly as an O part, so the last four nucleotides in the glycine-serine linker have been removed, as they will be regenerated by the O part 3' fusion site (TTCG), creating a scarless final assembly. To use this sequence in non-JUMP assembly, add TTCG to the end of the sequence.


Source

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References