Difference between revisions of "Part:BBa K4390016:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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This part was designed to be used in JUMP assembly as a N part, so the last four nucleotides in the cleavage site have been removed, as they will be regenerated by the N part 3' fusion site (AGCC), creating a scarless final assembly.
+
This part was designed to be used in JUMP assembly as a N part, so the last four nucleotides in the cleavage site have been removed, as they will be regenerated by the N part 3' fusion site (AGCC), creating a scarless final assembly. To use this sequence in non-JUMP assembly, add AGCC to the end of the sequence.
  
 
===Source===
 
===Source===

Revision as of 12:45, 7 August 2022


6xHis :: TEV protease cleavage site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to be used in JUMP assembly as a N part, so the last four nucleotides in the cleavage site have been removed, as they will be regenerated by the N part 3' fusion site (AGCC), creating a scarless final assembly. To use this sequence in non-JUMP assembly, add AGCC to the end of the sequence.

Source

-

References