Difference between revisions of "Part:BBa K091112:Experience"

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===User Reviews===
 
===User Reviews===
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The MWSU/Davdison iGEM 2009 team sequenced this promoter after obtaining gel verification results indicating that the part was too large. The team found that was a 33 bp insertion within this promoter directly before the suffix. We believe that the E.coli cells may have mutated the promoter to silence its activity in an effort to conserve energy and select against an attribute that did not necessarily improve its fitness.
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The MWSU/Davdison iGEM 2009 team sequenced this promoter after obtaining gel verification results indicating that the part was too large. The team found that there was a 35 bp insertion within this promoter directly before the suffix. We believe that the E.coli cells may have mutated the promoter to silence its activity in an effort to conserve energy and select against an attribute that did not necessarily improve its fitness. Here is the 35 bp insertion:
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TGTGTGGAATTGTGAGCGGATAACAATTTCACACA
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Revision as of 21:52, 20 July 2009

This part worked as expected in HB101 cells.
Trial1.png
Trial2.png


Applications of BBa_K091112

User Reviews

The MWSU/Davdison iGEM 2009 team sequenced this promoter after obtaining gel verification results indicating that the part was too large. The team found that there was a 35 bp insertion within this promoter directly before the suffix. We believe that the E.coli cells may have mutated the promoter to silence its activity in an effort to conserve energy and select against an attribute that did not necessarily improve its fitness. Here is the 35 bp insertion:

TGTGTGGAATTGTGAGCGGATAACAATTTCACACA


UNIQda1a8d57eb4c48ed-partinfo-00000000-QINU UNIQda1a8d57eb4c48ed-partinfo-00000001-QINU