Difference between revisions of "Part:BBa K091112:Experience"

Revision as of 21:47, 20 July 2009

This part worked as expected in HB101 cells.

Applications of BBa_K091112

User Reviews

The MWSU/Davdison iGEM 2009 team sequenced this promoter after obtaining gel verification results indicating that the part was too large. The team found that was a 33 bp insertion within this promoter directly before the suffix. We believe that the E.coli cells may have mutated the promoter to silence its activity in an effort to conserve energy and select against an attribute that did not necessarily improve its fitness.

UNIQe60aac307ec8dca4-partinfo-00000000-QINU UNIQe60aac307ec8dca4-partinfo-00000001-QINU

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 ===User Reviews===
-The MWSU/Davdison iGEM 2009 team sequenced this promoter after there was little to no expression using this it to express GFP and after obtaining what seemed like faulty results during gel verification of its size. The team found that there was a 33 bp insertion within this promoter before the suffix. We believe that the
+The MWSU/Davdison iGEM 2009 team sequenced this promoter after obtaining gel verification results indicating that the part was too large. The team found that was a 33 bp insertion within this promoter directly before the suffix. We believe that the E.coli cells may have mutated the promoter to silence its activity in an effort to conserve energy and select against an attribute that did not necessarily improve its fitness.
-E.coli cells may have mutated the promoter to silence its activity in an effort to conserve energy and selection against an attribute that did not necessarily improve its fitness.
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