Difference between revisions of "Part:BBa K4347007"
Victor5688 (Talk | contribs) |
Victor5688 (Talk | contribs) |
||
| Line 3: | Line 3: | ||
This part is an improvement from Bst Polymerase 1 (large fragment) for E.coli from the 2021 iGEM Fudan team: https://parts.igem.org/Part:BBa_K3790000. | This part is an improvement from Bst Polymerase 1 (large fragment) for E.coli from the 2021 iGEM Fudan team: https://parts.igem.org/Part:BBa_K3790000. | ||
| − | + | ||
| − | + | __TOC__ | |
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | |||
| + | Bst polymerase is a family I DNA polymerase derived from the thermophilic bacterium <em> Geobacillus stearothermophilus.</em> Bst polymerase is a unique polymerase | ||
| + | |||
| + | |||
How is bst used in LAMP, specific features of Bst that make it unique such that it lacks 3'-5' exonuclease etc... | How is bst used in LAMP, specific features of Bst that make it unique such that it lacks 3'-5' exonuclease etc... | ||
Revision as of 14:30, 11 July 2022
Bst with point mutations for enhanced thermal stability codon optimized for E.coli
This part is an improvement from Bst Polymerase 1 (large fragment) for E.coli from the 2021 iGEM Fudan team: https://parts.igem.org/Part:BBa_K3790000.
Usage and Biology
Bst polymerase is a family I DNA polymerase derived from the thermophilic bacterium Geobacillus stearothermophilus. Bst polymerase is a unique polymerase
How is bst used in LAMP, specific features of Bst that make it unique such that it lacks 3'-5' exonuclease etc...
Enhanced Thermal stability
Talk about the point mutations. Pictures of lab experiments
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 766
- 1000COMPATIBLE WITH RFC[1000]