Difference between revisions of "Part:BBa K4347011:Design"
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===Considerations===
 
===Considerations===
  
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section can be summary of
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Section can be summary from https://parts.igem.org/Part:BBa_K4347007# and https://parts.igem.org/Part:BBa_K4347010.
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-Considerations with point mutations.  
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Considerations with point mutations.  
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- Previous work that had already been done, heating mechanism of our device (might be more than 65 bc its a kettle based device), how fusion would interfere bst structure eg, alpha fold with phi29.  
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Previous work that had already been done, heating mechanism of our device (might be more than 65 bc its a kettle based device), how fusion would interfere bst structure eg, alpha fold with phi29.  
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===Source===
 
===Source===

Revision as of 15:56, 8 July 2022


Bst fusion with Sac7e and point mutations for enhanced thermal stability codon optimized for E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 5
    Illegal XhoI site found at 209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1015
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

Research that lead to choosing sac7e as ssb protien. Alpha fold analysis Yasara analysis

Considerations

Section can be summary from https://parts.igem.org/Part:BBa_K4347007# and https://parts.igem.org/Part:BBa_K4347010. -Considerations with point mutations. - Previous work that had already been done, heating mechanism of our device (might be more than 65 bc its a kettle based device), how fusion would interfere bst structure eg, alpha fold with phi29.

Source

Bst: https://parts.igem.org/Part:BBa_K3790000 Sac7e: https://parts.igem.org/Part:BBa_K4347006

References