Difference between revisions of "Part:BBa K4347007"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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How is bst used in LAMP, specific features of Bst that make it unique such that it lacks 3'-5' exonuclease etc... | How is bst used in LAMP, specific features of Bst that make it unique such that it lacks 3'-5' exonuclease etc... | ||
Revision as of 15:37, 8 July 2022
Bst with point mutations for enhanced thermal stability codon optimized for E.coli
small introduction about bst here... eg, what is it? what organism does it come from? Improvement from https://parts.igem.org/Part:BBa_K3790000 bst part from iGEM Fudon.
Usage and Biology
frbofbrhoi How is bst used in LAMP, specific features of Bst that make it unique such that it lacks 3'-5' exonuclease etc...
Enhanced Thermal stability
Talk about the point mutations. Pictures of lab experiments
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 766
- 1000COMPATIBLE WITH RFC[1000]