Difference between revisions of "Part:BBa J428100"

Revision as of 18:17, 5 July 2022

Interlab_NegC

Non-fluorescent negative control for InterLab studies 2022. It uses the same elements as the fluorescent constructs but expresses a non-fluorescent protein (LuxR). It includes a region upstream of the promoter to insulate transcription, a promoter (J23106), a RiboJ to insulate translation, the B0032 RBS, the CDS for the LuxR protein, and an efficient transcriptional terminator (rnpB_T1). It imposes the same burden on the bacteria for a more fair growth comparison between the different devices.

Specially designed for the Interlab_2022_DoubleConstructs and Interlab_2022_ThreeColors studies.

It can be used as negative control for fluorescent measurements in general.

Figure 1. SBOL Visual representation of the device J428100 from 2022 Interlab studies.

Interlab 2022 Experiment 1 Device

• Negative Control for Experiment 1 of the 2022 iGEM Interlab

The following device is used in the iGEM 2022 InterLaboratory Study for Experiment 1.

Interlab 2022 Experiment 2 Device

• Negative Control for Experiment 2 of the 2022 iGEM Interlab

The following device is used in the iGEM 2022 InterLaboratory Study for Experiment 2.

Usage and Biology

Sequence and Features