Difference between revisions of "Part:BBa J428100"

Revision as of 20:44, 8 June 2022

Interlab_NegC Non-fluorescent negative control for InterLab studies 2022. It uses the same elements as the fluorescent constructs but expresses a non-fluorescent protein (LuxR). It includes a region upstream of the promoter to insulate transcription, a promoter (J23106), a RiboJ to insulate translation, the B0032 RBS, the CDS for the LuxR protein, and an efficient transcriptional terminator (rnpb_T1). It imposes the same burden on the bacteria for a more fair growth comparison between the different devices.

Interlab_2022_DoubleConstructs and Interlab_2022_ThreeColors

Figure 1. SBOL Visual representation of the device J428100 from 2022 Interlab studies.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 71
Illegal NheI site found at 94
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_J428100 short</partinfo>
+Non-fluorescent negative control for InterLab studies 2022.
+It uses the same elements as the fluorescent constructs but expresses a non-fluorescent protein (LuxR).
+It includes a region upstream of the promoter to insulate transcription, a promoter (J23106), a RiboJ to insulate translation, the B0032 RBS, the CDS for the LuxR protein, and an efficient transcriptional terminator (rnpb_T1).
+It imposes the same burden on the bacteria for a more fair growth comparison between the different devices.
-One color negative control for Interlab_2022_DoubleConstructs and Interlab_2022_ThreeColors
+Interlab_2022_DoubleConstructs and Interlab_2022_ThreeColors
+[[File:EngCom_2022_J428100.png|500px|thumb|center|alt=J428100.|Figure 1. SBOL Visual representation of the device [https://parts.igem.org/Part:BBa_J428100 J428100] from [https://technology.igem.org/interlabs 2022 Interlab studies.] ]]
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