Difference between revisions of "Part:BBa K4421021"

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<partinfo>BBa_K4421021 short</partinfo>
 
<partinfo>BBa_K4421021 short</partinfo>
  
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This part is a CAR inducible promoter containing six NFAT-REs in a minimal IL-2 promoter to generate CAR response elements, which was placed upstream of the transcription factor Gal4-KRAB.In our engineered NK-92 cells, NFAT was coupled with KRAB activation after CAR stimulation.
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This part is a CAR inducible promoter containing six NFAT-RE sites in a minimal IL-2 promoter to generate CAR response elements, which was placed upstream of the transcription factor Gal4-KRAB.In our engineered NK-92 cells, NFAT was coupled with KRAB activation after CAR stimulation.
  
 
===Usage and Biology===
 
===Usage and Biology===
 
This part can serve as a downstream promotor of CAR recognition in synthetic immunology, especially in constructing CAR-classis cells with unique circus.Together with synnotch,we can really do a lot.  
 
This part can serve as a downstream promotor of CAR recognition in synthetic immunology, especially in constructing CAR-classis cells with unique circus.Together with synnotch,we can really do a lot.  
  
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===supplementary information===
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It consists of six NFAT-RE sites. NFAT-RE can bind with NFAT factors, thus activating  expression of the downstream genes, which is original from the IL-2 promotor.
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The regulation of IL-2 gene was revealed comprehensively during last century.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 06:02, 7 June 2022


NFAT-RE promotor

This part is a CAR inducible promoter containing six NFAT-RE sites in a minimal IL-2 promoter to generate CAR response elements, which was placed upstream of the transcription factor Gal4-KRAB.In our engineered NK-92 cells, NFAT was coupled with KRAB activation after CAR stimulation.

Usage and Biology

This part can serve as a downstream promotor of CAR recognition in synthetic immunology, especially in constructing CAR-classis cells with unique circus.Together with synnotch,we can really do a lot.

supplementary information

It consists of six NFAT-RE sites. NFAT-RE can bind with NFAT factors, thus activating expression of the downstream genes, which is original from the IL-2 promotor. The regulation of IL-2 gene was revealed comprehensively during last century. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]