Difference between revisions of "Part:BBa K4421021"
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This part is a CAR inducible promoter containing six NFAT-REs in a minimal IL-2 promoter to generate CAR response elements, which was placed upstream of the transcription factor Gal4-KRAB.In our engineered NK-92 cells, NFAT was coupled with KRAB activation after CAR stimulation. | This part is a CAR inducible promoter containing six NFAT-REs in a minimal IL-2 promoter to generate CAR response elements, which was placed upstream of the transcription factor Gal4-KRAB.In our engineered NK-92 cells, NFAT was coupled with KRAB activation after CAR stimulation. | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here> |
| − | + | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This part can serve as a downstream promotor of CAR stimulation in synthetic immunology, especially in constructing CAR-classis cells with unique circus.Together with synnotch,we can really do a lot. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 05:29, 7 June 2022
NFAT-RE promotor
This part is a CAR inducible promoter containing six NFAT-REs in a minimal IL-2 promoter to generate CAR response elements, which was placed upstream of the transcription factor Gal4-KRAB.In our engineered NK-92 cells, NFAT was coupled with KRAB activation after CAR stimulation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]