Difference between revisions of "Part:BBa K3725080"

 
Line 3: Line 3:
 
<partinfo>BBa_K3725080 short</partinfo>
 
<partinfo>BBa_K3725080 short</partinfo>
  
The Fusarium Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. In the presence of the <i>F. Oxysporum</i> FRP1 gene, the hairpin loop will unfold and express the downstream GFP reporter protein.  
+
The Fusarium Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. The sequence forms a hairpin loop, hiding machinery necessary for translation. However, in the presence of the FRP1 gene of <i>Fusarium oxysporum</i>, a plant pathogen, the hairpin loop unravels, allowing for expression of the downstream gene to occur. As a result, this sequence can be paired with a reporter gene to function as a biosensor for the presence of the plant disease <i>F. oxysporum</i>.
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 04:12, 18 December 2021


Fusarium Toehold Switch Sequence

The Fusarium Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. The sequence forms a hairpin loop, hiding machinery necessary for translation. However, in the presence of the FRP1 gene of Fusarium oxysporum, a plant pathogen, the hairpin loop unravels, allowing for expression of the downstream gene to occur. As a result, this sequence can be paired with a reporter gene to function as a biosensor for the presence of the plant disease F. oxysporum.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]