Difference between revisions of "Part:BBa K3725080"
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<partinfo>BBa_K3725080 short</partinfo> | <partinfo>BBa_K3725080 short</partinfo> | ||
− | The Fusarium Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. | + | The Fusarium Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. The sequence forms a hairpin loop, hiding machinery necessary for translation. However, in the presence of the FRP1 gene of <i>Fusarium oxysporum</i>, a plant pathogen, the hairpin loop unravels, allowing for expression of the downstream gene to occur. As a result, this sequence can be paired with a reporter gene to function as a biosensor for the presence of the plant disease <i>F. oxysporum</i>. |
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Latest revision as of 04:12, 18 December 2021
Fusarium Toehold Switch Sequence
The Fusarium Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. The sequence forms a hairpin loop, hiding machinery necessary for translation. However, in the presence of the FRP1 gene of Fusarium oxysporum, a plant pathogen, the hairpin loop unravels, allowing for expression of the downstream gene to occur. As a result, this sequence can be paired with a reporter gene to function as a biosensor for the presence of the plant disease F. oxysporum.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]