Difference between revisions of "Part:BBa K3725050"

Latest revision as of 03:47, 18 December 2021

Phytophthora Toehold Switch Sequence

The Phytophthora Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. The sequence forms a hairpin loop, hiding machinery necessary for translation. However, in the presence of the X24 gene of Phytophthora cryptogea, a plant pathogen, the hairpin loop unravels, allowing for expression of the downstream gene to occur. As a result, this sequence can be paired with a reporter gene to function as a biosensor for the presence of the plant disease P. cryptogea.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 03:47, 18 December 2021 (view source) Pranavkanthala (Talk \| contribs) ← Older edit		Latest revision as of 03:47, 18 December 2021 (view source) Pranavkanthala (Talk \| contribs)
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	<partinfo>BBa_K3725050 short</partinfo>		<partinfo>BBa_K3725050 short</partinfo>

−	The Phytophthora Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. The sequence forms a hairpin loop, hiding machinery necessary for translation. However, in the presence of the X24 gene of Phytophthora cryptogea, a plant pathogen, the hairpin loop unravels, allowing for expression of the downstream gene to occur. As a result, this sequence can be paired with a reporter gene to function as a biosensor for the presence of the plant disease P. cryptogea.	+	The Phytophthora Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. The sequence forms a hairpin loop, hiding machinery necessary for translation. However, in the presence of the X24 gene of <i>Phytophthora cryptogea</i>, a plant pathogen, the hairpin loop unravels, allowing for expression of the downstream gene to occur. As a result, this sequence can be paired with a reporter gene to function as a biosensor for the presence of the plant disease <i>P. cryptogea</i>.