Difference between revisions of "Part:BBa K3725050"
 
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<partinfo>BBa_K3725050 short</partinfo>
 
<partinfo>BBa_K3725050 short</partinfo>
  
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The Phytophthora Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. In the presence of the P. Cryptogea X24 gene, the hairpin loop will unfold and express the downstream GFP reporter protein.  
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The Phytophthora Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. The sequence forms a hairpin loop, hiding machinery necessary for translation. However, in the presence of the X24 gene of <i>Phytophthora cryptogea</i>, a plant pathogen, the hairpin loop unravels, allowing for expression of the downstream gene to occur. As a result, this sequence can be paired with a reporter gene to function as a biosensor for the presence of the plant disease <i>P. cryptogea</i>.
  
  

Latest revision as of 03:47, 18 December 2021


Phytophthora Toehold Switch Sequence

The Phytophthora Toehold Switch Sequence was derived from NUPACK and was constructed with the lowest normalized ensemble defect rate possible. The sequence forms a hairpin loop, hiding machinery necessary for translation. However, in the presence of the X24 gene of Phytophthora cryptogea, a plant pathogen, the hairpin loop unravels, allowing for expression of the downstream gene to occur. As a result, this sequence can be paired with a reporter gene to function as a biosensor for the presence of the plant disease P. cryptogea.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]