Difference between revisions of "Part:BBa K3725140"
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====DESIGN====
 
====DESIGN====
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In an attempt to increase the sensitivity of BBa_3725130 to extracellular phosphate, we designed part BBa_K3725150, a phosphate-sensitive biosensor with modified promoter BBa_K3725140 and GFP reporter. The new promoter utilizes a potentially more efficient consensus sequence by directly mutating the RNA polymerase binding site. The mutation affects the construct’s ability to transcribe downstream GFP, thus increasing the sensitivity of the construct and potentially creating an enhanced characterization curve.  
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In an attempt to increase the sensitivity of [https://parts.igem.org/Part:BBa_K3725130 BBa_K3725130] to extracellular phosphate, we designed part [https://parts.igem.org/Part:BBa_K3725150 BBa_K3725150], a phosphate-sensitive biosensor with modified promoter BBa_K3725140 and GFP reporter. The new promoter utilizes a potentially more efficient consensus sequence by directly mutating the RNA polymerase binding site. The mutation affects the construct’s ability to transcribe downstream GFP, thus increasing the sensitivity of the construct and potentially creating an enhanced characterization curve.  
 
   
 
   
  

Latest revision as of 20:49, 17 December 2021


Status: 500 Content-type: text/html

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DESIGN

In an attempt to increase the sensitivity of BBa_K3725130 to extracellular phosphate, we designed part BBa_K3725150, a phosphate-sensitive biosensor with modified promoter BBa_K3725140 and GFP reporter. The new promoter utilizes a potentially more efficient consensus sequence by directly mutating the RNA polymerase binding site. The mutation affects the construct’s ability to transcribe downstream GFP, thus increasing the sensitivity of the construct and potentially creating an enhanced characterization curve.


Status: 500 Content-type: text/html

Software error:

Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84.
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 84.
Global symbol "$groups" requires explicit package name at /websites/parts.igem.org/cgi/lib/User.pm line 85.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/IconBar.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 9.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8.
Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 12.
Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.