Difference between revisions of "Part:BBa K3725110"

Revision as of 20:28, 17 December 2021

PhoA Promoter with GFP Reporter

DESIGN

The inducible promoter BBa_K1682012, created by 2015 HKUST-Rice iGEM, was originally made to control the expression of the PhoA alkaline phosphatase gene following activation by the phosphorylated PhoB transcription factor.

CHARACTERIZATION

Utilizing the phosphate range of 0-100μM and characterization protocol (See: Modified Protocol), we characterized the BBa_K3725110 phosphate sensor. As seen in Figure, the characterization data yielded insignificant levels of fluorescence, thus leading us to conclude that the promoter is not sensitive enough to extracellular phosphate levels.

Figure 1. Characterization curve for BBa_K3725110 for phosphate concentrations between 0μM and 100μM.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 741

Revision as of 20:24, 17 December 2021 (view source) Manasvigupta (Talk \| contribs) (→‎EXPERIENCE) ← Older edit		Revision as of 20:28, 17 December 2021 (view source) Manasvigupta (Talk \| contribs) (→‎CHARACTERIZATION) Newer edit →
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	====CHARACTERIZATION====		====CHARACTERIZATION====
−	Utilizing the ~~modified~~ phosphate range and characterization protocol (See: [https://2021.igem.org/Team:Lambert_GA/Pho#:~:text=Engineering%20Success%20page.-,Characterization%20Protocol,-The%20failed%20characterization Modified Protocol]), we characterized the BBa_K3725110 phosphate sensor. ~~However~~, the characterization data ~~(Figure 1)~~ yielded insignificant levels of fluorescence, thus leading us to conclude that the promoter is not sensitive enough to extracellular phosphate levels.	+	Utilizing the phosphate range of 0-100μM and characterization protocol (See: [https://2021.igem.org/Team:Lambert_GA/Pho#:~:text=Engineering%20Success%20page.-,Characterization%20Protocol,-The%20failed%20characterization Modified Protocol]), we characterized the BBa_K3725110 phosphate sensor. As seen in Figure, the characterization data yielded insignificant levels of fluorescence, thus leading us to conclude that the promoter is not sensitive enough to extracellular phosphate levels.