Difference between revisions of "Part:BBa K4060105"
 
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<partinfo>BBa_K4060105 short</partinfo>
 
<partinfo>BBa_K4060105 short</partinfo>
  
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BBa_K4060101 with thermometer RBS
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<partinfo>BBa_K4060101</partinfo> with thermometer RBS
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The gene fragment Pre B2 is composed of Pre A2 and the Thermometer RBS <partinfo>BBa_K115002</partinfo>. We digested the vector fragment <partinfo>BBa_K115002</partinfo> with EcoRI and XbaI and the insert fragment Pre A2 with EcoRI and SpeI. We ligate these fragments together to form Pre B2.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:28, 7 December 2021


-- No description --

BBa_K4060101 with thermometer RBS

The gene fragment Pre B2 is composed of Pre A2 and the Thermometer RBS BBa_K115002. We digested the vector fragment BBa_K115002 with EcoRI and XbaI and the insert fragment Pre A2 with EcoRI and SpeI. We ligate these fragments together to form Pre B2.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]