Difference between revisions of "Part:BBa K4060105"
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<partinfo>BBa_K4060105 short</partinfo> | <partinfo>BBa_K4060105 short</partinfo> | ||
− | + | <partinfo>BBa_K4060101</partinfo> with thermometer RBS | |
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+ | The gene fragment Pre B2 is composed of Pre A2 and the Thermometer RBS <partinfo>BBa_K115002</partinfo>. We digested the vector fragment <partinfo>BBa_K115002</partinfo> with EcoRI and XbaI and the insert fragment Pre A2 with EcoRI and SpeI. We ligate these fragments together to form Pre B2. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 14:28, 7 December 2021
-- No description --
BBa_K4060101 with thermometer RBS
The gene fragment Pre B2 is composed of Pre A2 and the Thermometer RBS BBa_K115002. We digested the vector fragment BBa_K115002 with EcoRI and XbaI and the insert fragment Pre A2 with EcoRI and SpeI. We ligate these fragments together to form Pre B2.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]