Difference between revisions of "Part:BBa K4060101"
| (2 intermediate revisions by the same user not shown) | |||
| Line 3: | Line 3: | ||
<partinfo>BBa_K4060101 short</partinfo> | <partinfo>BBa_K4060101 short</partinfo> | ||
| − | a | + | The gene fragment Pre A2 is composed of pTetR <partinfo>BBa_R0040</partinfo> and a double terminator <partinfo>BBa_B0015</partinfo>. We digested the vector fragment BBa_R0040 with XbaI and EcoRI and the insert fragment <partinfo>BBa_B0015</partinfo> with EcoRI and SpeI. We ligated these fragments together to form Pre A2. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 14:24, 7 December 2021
[Pre A2] double terminator + pTetR
The gene fragment Pre A2 is composed of pTetR BBa_R0040 and a double terminator BBa_B0015. We digested the vector fragment BBa_R0040 with XbaI and EcoRI and the insert fragment BBa_B0015 with EcoRI and SpeI. We ligated these fragments together to form Pre A2.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]