Difference between revisions of "Part:BBa K4060104:Experience"

(Assembly of Pre B1 (pSB1C3))
Line 5: Line 5:
  
 
<html><body>
 
<html><body>
<img src="https://static.igem.org/mediawiki/parts/f/fb/Flocculation_with_inducers.png" height="475px" width="750px"/>
+
<img src="https://2021.igem.org/wiki/images/8/88/T--NYCU-Taipei--image9.png" height="475px" width="750px"/>
 
</body></html>
 
</body></html>
  
Fig.5 Agarose electrophoresis in order to verify Pre B1.
+
Fig.1 Agarose electrophoresis in order to verify Pre B1.
  
 
===Applications of BBa_K4060104===
 
===Applications of BBa_K4060104===

Revision as of 14:18, 7 December 2021


Assembly of Pre B1 (pSB1C3)

The gene fragment Pre B1 is composed of Pre A1 and the TetR gene BBa_C0040. We digested the vector fragment Pre A1 with SpeI and PstI; the insert fragment BBa_C0040 with XbaI and PstI. We ligated these fragments together to form Pre B1.

Fig.1 Agarose electrophoresis in order to verify Pre B1.

Applications of BBa_K4060104

User Reviews

UNIQ0ed1c5c73c8697e2-partinfo-00000003-QINU UNIQ0ed1c5c73c8697e2-partinfo-00000004-QINU