Difference between revisions of "Part:BBa K4060104:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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====Assembly of Pre B1 (pSB1C3)====
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how you used this part and how it worked out.
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The gene fragment Pre B1 is composed of Pre A1 and the TetR gene <partinfo>BBa_C0040</partinfo>. We digested the vector fragment Pre A1 with SpeI and PstI; the insert fragment <partinfo>BBa_C0040</partinfo> with XbaI and PstI. We ligated these fragments together to form Pre B1.
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<img src="https://static.igem.org/mediawiki/parts/f/fb/Flocculation_with_inducers.png" height="475px" width="750px"/>
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Fig.5 Agarose electrophoresis in order to verify Pre B1.
  
 
===Applications of BBa_K4060104===
 
===Applications of BBa_K4060104===

Revision as of 14:15, 7 December 2021


Assembly of Pre B1 (pSB1C3)

The gene fragment Pre B1 is composed of Pre A1 and the TetR gene BBa_C0040. We digested the vector fragment Pre A1 with SpeI and PstI; the insert fragment BBa_C0040 with XbaI and PstI. We ligated these fragments together to form Pre B1.

Fig.5 Agarose electrophoresis in order to verify Pre B1.

Applications of BBa_K4060104

User Reviews

UNIQbff2545466125103-partinfo-00000003-QINU UNIQbff2545466125103-partinfo-00000004-QINU