Difference between revisions of "Part:BBa K4060101:Experience"
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− | + | ====Assembly of Pre A2 (pSB1C3)==== | |
− | + | The gene fragment Pre A2 is composed of pTetR <partinfo>BBa_R0040</partinfo> and a double terminator <partinfo>BBa_B0015</partinfo>. We digested the vector fragment BBa_R0040 with XbaI and EcoRI and the insert fragment BBa_B0015 with EcoRI and SpeI. We ligated these fragments together to form Pre A2. | |
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+ | <img src="https://2021.igem.org/wiki/images/3/35/T--NYCU-Taipei--0A45820D-B05E-44B2-BD88-998A183211BB.png" height="475px" width="750px"/> | ||
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===Applications of BBa_K4060101=== | ===Applications of BBa_K4060101=== |
Revision as of 14:02, 7 December 2021
Assembly of Pre A2 (pSB1C3)
The gene fragment Pre A2 is composed of pTetR BBa_R0040 and a double terminator BBa_B0015. We digested the vector fragment BBa_R0040 with XbaI and EcoRI and the insert fragment BBa_B0015 with EcoRI and SpeI. We ligated these fragments together to form Pre A2.
Applications of BBa_K4060101
User Reviews
UNIQ29140a0f63f6ff22-partinfo-00000003-QINU UNIQ29140a0f63f6ff22-partinfo-00000004-QINU