Difference between revisions of "Part:BBa K4060001:Experience"
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Clairechen (Talk | contribs) (→1. Cloning MazE and MazF gene: The MazE and MAzF have been amplified by PCR from E. coli MG1655 genome DNA.) |
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| − | ==1. Cloning MazE and MazF gene: The MazE and MAzF have been amplified by PCR from E. coli MG1655 genome DNA.== | + | ====1. Cloning MazE and MazF gene: The MazE and MAzF have been amplified by PCR from E. coli MG1655 genome DNA.==== |
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<img src="https://2021.igem.org/wiki/images/6/6f/T--NYCU-Taipei--MazEMazF.png"height="300px" width="750px"/> | <img src="https://2021.igem.org/wiki/images/6/6f/T--NYCU-Taipei--MazEMazF.png"height="300px" width="750px"/> | ||
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Fig.1 Agarose electrophoresis in order to verify MazE and MazF genes. The purified DNA fragments had the expected length and were subsequently used for cloning. Lane 1, 100bp ladder. Lane 2, MazE PCR product. Lane 3 and 4, MazF PCR product. Land 5, 100bp ladder. | Fig.1 Agarose electrophoresis in order to verify MazE and MazF genes. The purified DNA fragments had the expected length and were subsequently used for cloning. Lane 1, 100bp ladder. Lane 2, MazE PCR product. Lane 3 and 4, MazF PCR product. Land 5, 100bp ladder. | ||
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==2. Gibson Assembly:== | ==2. Gibson Assembly:== | ||
Revision as of 13:28, 7 December 2021
1. Cloning MazE and MazF gene: The MazE and MAzF have been amplified by PCR from E. coli MG1655 genome DNA.
Fig.1 Agarose electrophoresis in order to verify MazE and MazF genes. The purified DNA fragments had the expected length and were subsequently used for cloning. Lane 1, 100bp ladder. Lane 2, MazE PCR product. Lane 3 and 4, MazF PCR product. Land 5, 100bp ladder.
2. Gibson Assembly:
Fig.2 Agarose electrophoresis in order to verify Construct 1 and Construct 2. Lane 1, 100bp ladder. Lane 1' partial of Life 1 kb Plus ladder. Lane 2 and 3, Construct 1 digested with BamHI. Lane 4, Construct 2 digested with KpnI and NdeI.
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