Difference between revisions of "Part:BBa K3792004"

Latest revision as of 03:39, 22 October 2021

BIOFAB apFAB98 Measurement Device

The apFAB98 Measurement Device is composed of a BIOFAB promoter, a ribosome binding site (BBa_B0034), mRFP1 (BBa_E1010), and a terminator (BBa_B0015). The measurement device was inserted into the pSB1K3 backbone using the NEB HiFi DNA Assembly method. The resulting plasmid vectors were used to transform the E. coli NEBExpress chassis. The promoter strength was measured via the amount of fluorescence detected in a flow cytometer. The level of fluorescence will be reported in molecules of equivalent PE-Texas Red (MEPTR) units. The analysis of the flow cytometry data is ongoing and will be shared during the Giant Jamboree. The interactive chart displayed below is a placeholder for the measurements.

Promoter Strength Measurement
Negative Control	Negative Control1000
apFAB40 BBa_K3792003	apFAB40 BBa_K37920031700
apFAB98 BBa_K3792004	apFAB98 BBa_K37920041700
apFAB51 BBa_K3792005	apFAB51 BBa_K37920051700

molecules of equivalent PE-Texas Red (MEPTR)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 615
Illegal AgeI site found at 727
1000
COMPATIBLE WITH RFC[1000]

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 <p>
-The apFAB98 Measurement Device is composed of a BIOFAB promoter, a ribosome binding site (BBa_B0034), mRFP1 (BBa_E1010), and a terminator (BBa_B0015). The measurement device was inserted into the pSB1K3 backbone using the NEB HiFi DNA Assembly method. The resulting plasmid vectors were used to transform the E. coli NEBExpress chassis. The promoter strengths were measured via the amount of fluorescence detected in a flow cytometer. The levels of fluorescence will be reported in molecules of equivalent PE-Texas Red (MEPTR) units. <strong>The analysis of the flow cytometry data is ongoing and will be shared during the Giant Jamboree.  The interactive chart displayed below is a placeholder for the measurements</strong>.
+The apFAB98 Measurement Device is composed of a BIOFAB promoter, a ribosome binding site (BBa_B0034), mRFP1 (BBa_E1010), and a terminator (BBa_B0015). The measurement device was inserted into the pSB1K3 backbone using the NEB HiFi DNA Assembly method. The resulting plasmid vectors were used to transform the E. coli NEBExpress chassis. The promoter strength was measured via the amount of fluorescence detected in a flow cytometer. The level of fluorescence will be reported in molecules of equivalent PE-Texas Red (MEPTR) units. <strong>The analysis of the flow cytometry data is ongoing and will be shared during the Giant Jamboree. The interactive chart displayed below is a placeholder for the measurements</strong>.
 </p>