Difference between revisions of "Part:BBa K3792004"
 
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__NOTOC__
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{{FSU/ChartsCSS}}
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<partinfo>BBa_K3792004 short</partinfo>
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apFAB98 is a weak promoter taken from the BIOFAB collection.  
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<html>
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<style>
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#modular-promoter-library-chart.column {
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  height: 250px;
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  max-width: 50% !important;
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  margin: 0 auto;
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  --labels-size: 2rem;
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}
  
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This device uses apFAB98 to express mRFP1, a red fluorescent protein. The apFAB98 test device consists of apFAB98, an RBS, the coding sequence for mRFP1, and a terminator.
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#modular-promoter-library-chart tr {
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  transition-duration: 0.3s;
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}
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#modular-promoter-library-chart tr:hover {
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  background-color: rgba(0, 0, 0, 0.2);
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}
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#modular-promoter-library-chart tr:hover th {
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  background-color: rgba(0, 0, 0, 0.4);
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  color: #fff;
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}
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#my-chart {
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  display: grid;
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  align-items: center;
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  justify-items: center;
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  grid-template-columns: 50px 1fr;
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  grid-template-rows: 250px 50px;
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  grid-template-areas:
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    "data-axis chart"
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    ".        primary-axis";
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}
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#my-chart > table {
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  grid-area: chart;
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}
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#my-chart > .primary-axis {
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  grid-area: primary-axis;
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}
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#my-chart > .data-axis {
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  grid-area: data-axis;
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  writing-mode: tb-rl;
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  transform: rotateZ(180deg);
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}
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</style>
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</html>
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__NOTOC__
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<partinfo>BBa_K3792004 short</partinfo>
  
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This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEB 5 alpha.
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<p>
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The apFAB98 Measurement Device is composed of a BIOFAB promoter, a ribosome binding site (BBa_B0034), mRFP1 (BBa_E1010), and a terminator (BBa_B0015). The measurement device was inserted into the pSB1K3 backbone using the NEB HiFi DNA Assembly method. The resulting plasmid vectors were used to transform the E. coli NEBExpress chassis. The promoter strength was measured via the amount of fluorescence detected in a flow cytometer. The level of fluorescence will be reported in molecules of equivalent PE-Texas Red (MEPTR) units. <strong>The analysis of the flow cytometry data is ongoing and will be shared during the Giant Jamboree. The interactive chart displayed below is a placeholder for the measurements</strong>.
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</p>
  
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The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.
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<div id="my-chart">
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<table id="modular-promoter-library-chart" class="charts-css column show-labels show-data show-primary-axis show-10-secondary-axes">
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      <caption>Promoter Strength Measurement</caption>
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      <tr>
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        <th scope="row">Negative<br>Control</th>
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        <td style="--size: calc(1000/2000)"><span class="tooltip">Negative Control</span><span class="data">1000</span></td>
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      </tr>
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      <tr>
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      <th scope="row">apFAB40<br>BBa_K3792003</th>
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      <td style="--size: calc(1700/2000)"><span class="tooltip">apFAB40<br>BBa_K3792003</span><span class="data">1700</span></td>
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      </tr>
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      <tr>
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        <th scope="row">apFAB98<br>BBa_K3792004</th>
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        <td style="--size: calc(1700/2000)"><span class="tooltip">apFAB98<br>BBa_K3792004</span><span class="data">1700</span></td>
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      </tr>
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      <tr>
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        <th scope="row">apFAB51<br>BBa_K3792005</th>     
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        <td style="--size: calc(1700/2000)"><span class="tooltip">apFAB51<br>BBa_K3792005</span><span class="data">1700</span></td>
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    </tr>
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  </table>
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  <div class="data-axis">molecules of equivalent PE-Texas Red (MEPTR)</div>
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</div>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:39, 22 October 2021


BIOFAB apFAB98 Measurement Device

The apFAB98 Measurement Device is composed of a BIOFAB promoter, a ribosome binding site (BBa_B0034), mRFP1 (BBa_E1010), and a terminator (BBa_B0015). The measurement device was inserted into the pSB1K3 backbone using the NEB HiFi DNA Assembly method. The resulting plasmid vectors were used to transform the E. coli NEBExpress chassis. The promoter strength was measured via the amount of fluorescence detected in a flow cytometer. The level of fluorescence will be reported in molecules of equivalent PE-Texas Red (MEPTR) units. The analysis of the flow cytometry data is ongoing and will be shared during the Giant Jamboree. The interactive chart displayed below is a placeholder for the measurements.

Promoter Strength Measurement
Negative
Control		 Negative Control1000
apFAB40
BBa_K3792003		 apFAB40
BBa_K37920031700
apFAB98
BBa_K3792004		 apFAB98
BBa_K37920041700
apFAB51
BBa_K3792005		 apFAB51
BBa_K37920051700
molecules of equivalent PE-Texas Red (MEPTR)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 615
    Illegal AgeI site found at 727
  • 1000
    COMPATIBLE WITH RFC[1000]